[Preparation of the polypeptide antibody against human transcriptional factors TFDP1 and TFDP3].

AIM To prepare the polyclonal antibody against human transcriptional factors DP3(TFDP3) and DP1(TFDP1) and identify their reactivity and specificity. METHODS Two totally different peptides between TFDP1 and TFDP3 were chosen as antigens to inoculate rabbits. The proteins from HEK293 cells were transiently transfcted with eukaryotic expression vectors of… CONTINUE READING