[Preparation of polyclonal antibody to human prion protein using the expressed GST-PrP fusion protein as antigen].

Abstract

OBJECTIVE Preparing specific antibody to prion protein. METHODS Using prokaryotic expressed GST-PrP fusion protein as antigen, rabbits were immunized subcutaneously. RESULTS ELISA assay revealed that the titer of the prepared antiserum against expressed PrP protein was as high as 1:128 000. Western blot test showed that the antiserum was able to react… (More)

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@article{Zhao2000PreparationOP, title={[Preparation of polyclonal antibody to human prion protein using the expressed GST-PrP fusion protein as antigen].}, author={Xiaodong Zhao and X. Dong and Wu Zhou}, journal={Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology}, year={2000}, volume={14 2}, pages={131-3} }