[Preparation of monoclonal antibodies against the adhesion protein 33 of Trichomonas vaginalis].

@article{Huang2007PreparationOM,
  title={[Preparation of monoclonal antibodies against the adhesion protein 33 of Trichomonas vaginalis].},
  author={Hui-cong Huang and S Yu and Ming Cai and Feng Tan and Xiao-yun Zheng and Chang-wang Pan},
  journal={Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases},
  year={2007},
  volume={25 2},
  pages={97-100, 105}
}
OBJECTIVE To prepare and characterize the monoclonal antibodies (McAbs) against recombinant adhesion protein 33 (AP33) of Trichomonas vaginalis. METHODS The purified recombinant fusion protein AP33 was used as antigen to immunize BALB/c mice. Sp2/0 myeloma cells were fused with the splenocytes from immunized BALB/c mice. After ELISA screening and 4 times of limited dilution, 5 positive hybridoma cell lines were obtained, and the biological properties of the McAbs were identified by Western… CONTINUE READING