[Preparation of monoclonal antibodies against hFGF-21 and identification of epitope].

Abstract

OBJECTIVE To prepare monoclonal antibodies (mAbs) against human fibroblast growth factor 21 (hFGF-21), and identify the epitope of hFGF-21 mAb through bacterial display. METHODS With hFGF-21 as an immunogen and detective antigen, we screened hybridoma cell lines secreting anti-hFGF-21 mAbs using indirect ELISA. The different fragments of hFGF-21 were… (More)

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