[Preparation, characterization and preliminary application of recombinant protein AP33 of trichomonas vaginalis].

OBJECTIVE To clone ap33 gene of Trichomonas vaginalis( T. v), construct prokaryotic expression system of the gene and identify its antigenicity and immunogenicity. METHODS The total RNA was extracted from a clinical isolate Tv317 and the cDNA was synthesized by reverse transcription. The ap33 gene from cDNA of Tv317 was amplified by PCR. The nucleotide… CONTINUE READING