[Optimization of cloning and expression of beta-glucanase gene from Bacillus amyloliquefaciens].

@article{Li2009OptimizationOC,
  title={[Optimization of cloning and expression of beta-glucanase gene from Bacillus amyloliquefaciens].},
  author={Yongxian Li and Yan Xie and Linjiang Zhu and Yixin Zhang and Guoxian Gu and Qi Li},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2009},
  volume={25 4},
  pages={
          542-8
        }
}
To compare of performance of beta-1,3-1,4-glucanase gene (bgl) in different expression systems, the beta-1,3-1,4-glucanase gene (GenBank Accession No. EU623974) was amplified from Bacillus amyloliquefaciens BS5582 by PCR and was cloned into three vectors pEGX-4T-1, pET20b(+) and pET28a(+) to construct pEGX-4T-1-bgl, pET20b(+)-bgl and pET28a(+)-bgl recombinant plasmids. The pEGX-4T-1-bgl was transformed into three different Escherichia coli host strains. The pET20b (+)-bgl and pET28a (+)-bgl… CONTINUE READING

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