[Optimization of a spectrophotometry assay of total and oxidized blood glutathione: comparison with a fluorimetric method].

Abstract

We developed a method for the enzymatic assay of glutathione which is easy to practice, rapid, specific, based on the reaction of the thiol group of glutathione with dithiobis-nitrobenzoic acid after the action of glutathione reductase in the presence of NADPH. This spectrophotometric technique allowed, on the one hand, the determination of total… (More)

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