[On-column refolding and purification of human EGF receptor L2 domain inclusion body overexpressed in Escherichia coli].

@article{Xu2005OncolumnRA,
  title={[On-column refolding and purification of human EGF receptor L2 domain inclusion body overexpressed in Escherichia coli].},
  author={Li-hui Xu and A X Hong and Xian-hui He},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2005},
  volume={21 4},
  pages={597-603}
}
The human epidermal growth factor receptor (EGFR) extracellular region (residues 1-621) consists of four subdomains, i.e. L1, S1, L2, and S2. The L2 domain (EGFR-L2) is composed of residues 311-479 and plays a major role in ligand-binding. Due to the high content of cysteine residues (42 cysteines) in the S1 and S2 domains, it is quite difficulty to get a correctly refolded product of the complete EGFR extracellular domain. In contrast, only 4 cysteine residues are present in EGFR-L2 domain… CONTINUE READING