[Obtention, purification and characterization of IgY polyclonal antibodies, developed in hens, directed against Colombian isolates of Giardia duodenalis].

@article{Garca2005ObtentionPA,
  title={[Obtention, purification and characterization of IgY polyclonal antibodies, developed in hens, directed against Colombian isolates of Giardia duodenalis].},
  author={Dabeiba Adriana Garc{\'i}a and Rub{\'e}n Santiago Nicholls and Adriana Ar{\'e}valo and Orlando Torres and Sof{\'i}a Duque},
  journal={Biomedica : revista del Instituto Nacional de Salud},
  year={2005},
  volume={25 4},
  pages={451-63}
}
INTRODUCTION The development of polyclonal antibodies requires laboratory animals, as the usually rabbits, which are bled for obtaining the immunoglobulins. Hens are an alternative to developing IgY polyclonal antibodies. OBJECTIVE In the current study, hens were used to develop IgY anti-Giardia duodenalis antibodies and to evaluate five protocols for its purification. MATERIALS AND METHODS Three hens were immunized intramuscularly immunized with Colombian Giardia duodenalis isolates… CONTINUE READING