[Nuclease isoforms of natural and recombinant strains of Serratia marcescens. Comparative characteristics of plasma desorption mass spectrometry].

@article{Pedersen1995NucleaseIO,
  title={[Nuclease isoforms of natural and recombinant strains of Serratia marcescens. Comparative characteristics of plasma desorption mass spectrometry].},
  author={Jakob R Pedersen and Maria N. Filimonova and Peter Roepstorff and Katrin Biedermann},
  journal={Biokhimiia},
  year={1995},
  volume={60 3},
  pages={450-61}
}
The primary structure of isoforms of natural nuclease secreted by Serratia marcescens as well as of recombinant nuclease produced by Escherichia coli has been characterized by plasma desorption mass spectrometry. The isoforms were isolated from the purified nuclease on a DEAE-cellulose anion-exchange column and digested with endoproteinase Lys-C. The peptides generated were isolated by reversed phase IIPLC and their molecular masses determined by plasma desorption mass spectrometry. Among the… CONTINUE READING