The native oligomers of histones isolated from calf thymus nuclear chromatin were investigated. After mild treatment with trypsin, the tetramer, (H3-H4)2, has a molecular weight of 36 000, whereas Mr of the dimer, H2a-H2b, is equal to 25 000. Intact oligomers have Mr of 55 000 (tetramer) and 33 000 (dimer). Analysis of the fluorescence intensity changes… (More)
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