[Molecular cloning and DNA sequencing of OspC gene of two strains Borrelia burgdorferi isolated in China].


OBJECTIVE To investigate the variation of OspC gene in two Chinese isolates of Borrelia burgdorferi. METHODS PCR technique was used to amplify the OspC gene from the whole cellular DNA of isolates BT01 and BJ-9011. The amplified products were inserted into plasmid pGEM-3ZF(+) and sequenced. RESULTS Except the signal peptide, the OspC genes of the two… (More)


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