[Modifying methylase SsoI from Shigella sonnei 47 cells].

Abstract

A simple and fast method for isolation and purification of SsoI methylase from the bacterial strain Shigella sonnei 47 has been proposed. The enzyme is a modifying component of host cell specificity system and protects the acceptor DNA from hydrolysis by restriction endonucleases SsoI and EcoRI. The method is based on the hydrophobic chromatography of… (More)

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