[Micromethod of determining phosphatase activity of staphylococci].

Abstract

A rapid method for measuring phosphatase activity of staphylococci has been developed. Russian 96-well polystyrene plates are used. 0.1 ml of 0.2% sodium phenolphthalein phosphate solution in 1% peptone water, pH 6.8 to 7.0, and 0.1 ml of the tested strain suspension in normal saline are put into wells and incubated for 5 h at 37 degrees C. The results are… (More)

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