• Corpus ID: 11667151

[Metabolic engineering of wild acid-resistant yeast for L-lactic acid production].

@article{Zhang2011MetabolicEO,
  title={[Metabolic engineering of wild acid-resistant yeast for L-lactic acid production].},
  author={Qin Zhang and Liang Zhang and Zhongyang Ding and Zhengxiang Wang and Guiyang Shi},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2011},
  volume={27 7},
  pages={
          1024-31
        }
}

• Qin Zhang, Liang Zhang, +2 authors G. Shi
• Published 1 July 2011
• Chemistry, Medicine
• Sheng wu gong cheng xue bao = Chinese journal of biotechnology

In order to obtain a yeast strain able to produce L-lactic acid under the condition of low pH and high lactate content, one wild acid-resistant yeast strain isolated from natural samples, was found to be able to grow well in YEPD medium (20 g/L glucose, 20 g/L tryptone, 10 g/L yeast extract, adjusted pH 2.5 with lactic acid) without consuming lactic acid. Based on further molecular biological tests, the strain was identified as Candida magnolia. Then, the gene ldhA, encoding a lactate dehydrogenase from Rhizopus oryzae, was cloned into a yeast shuttle vector containing G418 resistance gene. The resultant plasmid pYX212-kanMX-ldhA was introduced into C. magnolia by electroporation method. Subsequently, a recombinant L-lactic acid producing yeast C. magnolia-2 was obtained. The optimum pH of the recombinant yeast is 3.5 for lactic acid production. Moreover, the recombinant strain could grow well and produce lactic acid at pH 2.5. This recombinant yeast strain could be useful for producing L-lactic acid.