[Lipid peroxidation, production of PGE2 and cellular mortality induced by UV in cultured human skin fibroblasts].

@article{Filipe1995LipidPP,
  title={[Lipid peroxidation, production of PGE2 and cellular mortality induced by UV in cultured human skin fibroblasts].},
  author={Paulo Manuel Filipe and Jo{\~a}o Pedro Rego Freitas and Marta Garc{\'i}a de Castro and Rodrigo Klaassen Pinto and Afonso Camilo Fernandes and L Cirne de Castro and Teresa Mart{\'i}n Rico and Francisco G. F. Rodrigo},
  journal={Comptes rendus des seances de la Societe de biologie et de ses filiales},
  year={1995},
  volume={189 3},
  pages={
          443-51
        }
}
UV irradiation induces lipid peroxidation (LPO) and cell damage. The aim of the present work was the study of UVB radiation effects on cultured human skin fibroblasts, concerning LPO, prostaglandine E2 (PGE2) formation and cell viability. The cells were exposed to 50, 100, and 150 mJ/cm2 of UVB irradiation. Cellular TBARS and supernatant fluorescent substances were measured spectrofluorimetrically. PGE2 was measured using an immunoenzymatic method. Cell viability was evaluated by the MTT test… CONTINUE READING