[Isolation of monoclonal antibodies to antigen Lyt-3.2].

  title={[Isolation of monoclonal antibodies to antigen Lyt-3.2].},
  author={Alexander V Filatov and A. V. Chervonskii and B. D. Brondz},
  journal={Biulleten' eksperimental'noi biologii i meditsiny},
  volume={98 8},
A hybridoma producing monoclonal antibodies (McAb) NATF9.9 (F9) was obtained from fusion of murine myeloma X63 and splenocytes of AKR mice immunized with a single intravenous injection of 5 X 10(7) thymocytes of CBA mice. F9 McAb were cytotoxic for 80% thymocytes, 10% splenocytes, 20% lymph node cells, 85% cortical and 32% medullary thymocytes of CBA, C57BL/6, BALB/c, DBA/2 and SJL but not for the cells of C58 and AKR mice. F9 McAb reacted only with T cells and did not react with B cells and… CONTINUE READING

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