[Isolation of chlamydophila psittaci from avian samples and establishment of a respiratory infection murine model].

@article{Tang2010IsolationOC,
  title={[Isolation of chlamydophila psittaci from avian samples and establishment of a respiratory infection murine model].},
  author={Guofang Tang and Lili Chen and Liangzhuan Liu and Zhongyu Li and Shaosheng Wang and Lei Xu and Yimou Wu},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2010},
  volume={50 12},
  pages={1657-63}
}
OBJECTIVE To optimize the isolation and culture technique of Chlamydophila psittaci avian strains and to establish an animal model infected with C. psittaci. METHODS C. psittaci ompA gene was amplified from DNA extracted from bird livers by polymerase chain reactions (PCR). For the PCR positive avian samples, the liver tissues were homogenized and used to incubated with HeLa or Vero cell monolayers for 72 h in different dilutions, and chlamydia inclusion bodies were detected by… CONTINUE READING