[Isolation and identification of DNA fragments frequently deleted from tumor cell lines of human esophageal cancer].

@article{Hou1997IsolationAI,
  title={[Isolation and identification of DNA fragments frequently deleted from tumor cell lines of human esophageal cancer].},
  author={Pi-yong Hou and Zhao-wen Wang and Xiao-hu Xu},
  journal={Zhonghua yi xue za zhi},
  year={1997},
  volume={77 10},
  pages={737-41}
}
OBJECTIVE To clone tumor suppressor genes associated with human esophageal cancer (EC). METHODS DNA fragments deleted from cell lines of esophageal cancer EC8712 and EC8733 were isolated by using modified genomic substractive hybridization. Twenty EC tissues paired with adjacent normal mucosa were analysed by Southern blot and PCR with these fragments as probes. RESULTS Seven DNA fragments deleted from EC cell lines obtained were named as 12H1, 12H2, 33H3, 33H4, 12B1, 33B2 and 33B3… CONTINUE READING