[Isolation and characterisation of continuous human embryonic stem cell lines].

@article{Krylova2003IsolationAC,
  title={[Isolation and characterisation of continuous human embryonic stem cell lines].},
  author={Tatiana Krylova and Valery V. Zenin and N S Musorina and Vladislav S. Baranov and N. K. Bichevaia and V. S. Korsak and N. N. Nikol'skii and George P. Pinaev and G G Polianskaia},
  journal={Tsitologiia},
  year={2003},
  volume={45 12},
  pages={1172-8}
}
A long-term cultivation (5-8 months) of human blastocyst-derived embryonic cells (hES) was performed. Several properties of hESs were examined to prove the state of continuous cell lines. These cells have passed through 100-175 population doublings with the average population doubling time equal to 37.0 +/- 1.5 h. Isolated hESs, referred to as HESC-1, HESC-2, HESC-3, HESC-4, cultivated on mitotically inactivated mouse embryonic fibroblasts (STO continuous cell line), formed multilayer colonies… CONTINUE READING