[Isolation, purification and properties of restriction endonuclease EcoRII].

@article{Kosykh1982IsolationPA,
  title={[Isolation, purification and properties of restriction endonuclease EcoRII].},
  author={V. G. Kosykh and S. A. Puntezhis and Ia I Bur'ianov and Alexander A. Baev},
  journal={Biokhimiia},
  year={1982},
  volume={47 4},
  pages={
          619-25
        }
}
The restriction endonuclease Eco RII was isolated and purified to homogeneity. The isolation procedure involved the use of the E. coli strain B834/pSK323, containing the recombinant plasmide pSK323 which provides for the oversynthesis of Eco RII enzymes. Data from gel filtration and Na-DS electrophoresis suggest that the restriction endonuclease Eco RII is a protein made up of two subunits, each with molecular weight of 44 000. 

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