[Inverse PCR amplification of the complete major capsid protein gene of lymphocystis disease virus isolated from Rachycentron canadum and the phylogenetic analysis of the virus].

@article{Fu2007InversePA,
  title={[Inverse PCR amplification of the complete major capsid protein gene of lymphocystis disease virus isolated from Rachycentron canadum and the phylogenetic analysis of the virus].},
  author={Xiao-zhe Fu and Cun-bin Shi and Ning-qiu Li and Hou-jun Pan and Ou-Qin Chang and Shu-qin Wu},
  journal={Bing du xue bao = Chinese journal of virology},
  year={2007},
  volume={23 5},
  pages={412-6}
}
The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum (LCDV-rc) was amplified and analysed. The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses, then the flaking sequences adjacent to the core region were amplified by inverse PCR, and the complete sequence was obtained by combining all of them. The open reading frame of the gene is 1380bp in length, encoding a putative… CONTINUE READING

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