[Initial study on zili functions to the development of the germ layers during zebrafish early embryogenesis].


OBJECTIVE To study the role for piwil2 gene (zili) in the development of the ectoderm, mesoderm and endoderm during early embryogenesis of zebrafish. METHODS zili morpholino antisense oligonucleotide and 5 mis-pair control morpholino were used in this study. zili was cloned into expression vector. zili mRNA was synthesized in vitro. The antisense RNA… (More)


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