• Corpus ID: 34384701

[In vivo and in vitro protection against lethal activity of Buthus occitanus tunetanus venom with a recombinant protein].

@article{Benkhadir2002InVA,
  title={[In vivo and in vitro protection against lethal activity of Buthus occitanus tunetanus venom with a recombinant protein].},
  author={Khadija Benkhadir and Thouraya Mejri and Rahima Bel Haj Rhouma and Mohamed El Ayeb and Habib Karoui},
  journal={Archives de l'Institut Pasteur de Tunis},
  year={2002},
  volume={79 1-4},
  pages={
          19-26
        }
}
We report the use of recombinant scorpion toxin in the form of fusion protein as antigen for mice immunisation. The aim is to produce protective antisera against lethal activity of the venom from Tunisian scorpion Buthus occitanus tunetanus, responsible for several annually reported human cases of scorpion stings. The gene encoding Bot III (the most toxic alpha toxin of Buthus occitanus tunetanus) was fused to the sequence encoding synthetic ZZ domains of staphylococcal protein A. The construct… 
4 Citations

Evolution of alternative methodologies of scorpion antivenoms production.

Innovative Immunization Strategies for Antivenom Development

All reported studies examining venom-independent innovative immunization strategies for antivenom development are compiled, including a brief description of toxin families of medical relevance found in snake, scorpion, and spider venoms is presented, as well as how biochemical, bioinformatic, and omics tools could aid the development of next-generation antivenoms.

Biotechnological Trends in Spider and Scorpion Antivenom Development

The increasing number of discovery and development efforts within development of spider and scorpion antitoxins based on small molecules, antibodies and fragments thereof, and next generation immunization strategies may point towards an upcoming transition from serum-based antivenoms towards therapeutic solutions based on modern biotechnology.