[In vitro cultivation model of Cryptosporidium parvum in MDCK cells and its development].

Abstract

OBJECTIVE To develop an in vitro culture system for Cryptosporidium parvum in Madin-Darby canine kidney (MDCK) cell and observe its life cycle (from desquamate to oocyst). METHODS Oocysts of C. parvum were co-cultured with MDCK cells in vitro. Culture condition was optimized and the life cycle of C.parvum investigated. RESULTS The optimal culture… (More)

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