[Immunocytochemical analysis of interleukin-1 beta production by human monocytes].

@article{Simbirtsev1991ImmunocytochemicalAO,
  title={[Immunocytochemical analysis of interleukin-1 beta production by human monocytes].},
  author={Andrey Semenovich Simbirtsev and Velentina G Konusova and S. A. Ketlinskii},
  journal={Biulleten' eksperimental'noi biologii i meditsiny},
  year={1991},
  volume={112 9},
  pages={278-80}
}
IL-1 was localized within the cytoplasm of human blood monocytes by indirect immunofluorescence using polyclonal rabbit antibodies. After LPS stimulation first IL-1-positive cells appeared at 2 hours and maximal intracellular IL-1 concentration was observed at 10-24 hours when nearly 90% monocytes were labeled with subsequent decline at 48 hours. The… CONTINUE READING