[Hairy-root culture of Polygonium multiflorum Thunb. and the production of its active constituents-anthraquinones].

@article{Wang2002HairyrootCO,
  title={[Hairy-root culture of Polygonium multiflorum Thunb. and the production of its active constituents-anthraquinones].},
  author={Li Hong Wang and Rong-min Yu and Hui Bin Zhang and K. -J. Cheng},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2002},
  volume={18 1},
  pages={69-73}
}
Transformed hairy roots of Polygonium multiflorum Thunb. were obtained by the transformation of Agrobacterium rhizogenes LBA9402. It was clearly demonstrated that T-DNA of A. Rhizogenes Ri plasmid was integrated into the cells of hairy roots by the experiments of PCR and Southern hybridization. Through the screen of basic medium and the research of growth curve of hairy roots, the optimum inoculum time was selected in 30 days or so in the optimum condition-MS medium. On the condition, the… CONTINUE READING

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