[Genotyping of hepatitis E virus by PCR combining with single restriction endonuclease analysis].

@article{Pan2005GenotypingOH,
  title={[Genotyping of hepatitis E virus by PCR combining with single restriction endonuclease analysis].},
  author={N Pan and Xing Dai and Ji-hong Meng and She-lan Liu},
  journal={Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology},
  year={2005},
  volume={19 2},
  pages={179-81}
}
OBJECTIVE To develop a simple method for genotyping of hepatitis E virus (HEV) and to investigate HEV genotype distribution in Nanjing area. METHODS Twenty-seven full HEV sequences currently-available in GenBank were analyzed with MegAlign and MapDraw programs of DNA STAR software. Degenerate primers were designed and applied to amplify a fragment in HEV ORF1 region. HEV genotypes were determined by the size of the PCR products and by single restriction endonuclease analysis. RESULTS The… CONTINUE READING