[Genetic transformation of the fungus Gliocladium sp. mediated by Agrobacterium tumefaciens].

@article{Wang2013GeneticTO,
  title={[Genetic transformation of the fungus Gliocladium sp. mediated by Agrobacterium tumefaciens].},
  author={Yanling Wang and Pengjie Hu and Erwei Li and Xingzhong Liu and Yongsheng Che and Gang Liu},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2013},
  volume={53 11},
  pages={1233-9}
}
OBJECTIVE To construct a transformation system in Gliocladium sp. 6.102, a Cordyceps-colonizing fungus producing a variety of epipolythiodioxopiperazine (ETP) compounds with drug potentials. METHODS Agrobacterium tumefaciens-mediated transformation (ATMT) was used to transform Gliocladium sp. 6.102. The factors of bacterial cell concentration, co-cultivation time, pH and acetosyringone concentration were optimized. RESULTS; A total of 50 -100 transformants per 10(6) fungal conidia was… CONTINUE READING