[Gene transfer of tumor necrosis factor related apoptosis inducing ligand to endothelial progenitor cell for ovarian cancer therapy].

@article{Zhou2005GeneTO,
  title={[Gene transfer of tumor necrosis factor related apoptosis inducing ligand to endothelial progenitor cell for ovarian cancer therapy].},
  author={Li Na Zhou and You-ji Feng and Ming-wei Zhu and Wei Zhang and Hui-ming Jin},
  journal={Zhonghua fu chan ke za zhi},
  year={2005},
  volume={40 12},
  pages={822-5}
}
OBJECTIVE To express tumor necrosis factor related apoptosis inducing ligand (TRAIL) protein using endothelial progenitor cell (EPC) and observe antitumor activity of secreted TRAIL in cell culture supernatant. METHODS EPC were isolated by immunomagnetic sorting. Human TRAIL plasmid was transfected into EPC by lipofectamine 2000. The cell culture supernatant was harvested and the secreted protein was measured by immunoassay method. Apoptosis rate was analysed by flow cytometry. RESULTS EPC… CONTINUE READING