• Medicine, Biology
  • Published in
    Wei sheng wu xue bao = Acta…
    2010

[Gene cloning, codon optimization and functional expression of Yarrawia lipolytica lipase Lip1].

@article{Zhang2010GeneCC,
  title={[Gene cloning, codon optimization and functional expression of Yarrawia lipolytica lipase Lip1].},
  author={Li Zhang and Heyun Zhao and Li Ling Xu and Yun Liu and Yunjun Yan},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2010},
  volume={50 7},
  pages={
          969-74
        }
}
OBJECTIVE To implement inducible and constitutive over-expression of Yarrowia lipolytica lipase gene lipl in Pichia pastoris using codon optimization. METHODS We cloned Y. lipolytica lipase gene lip1 according to codon bias of P. pastoris, and optimized lipl using overlap extension PCR synthesis. Then, we cloned the original and optimized genes into the induced vector pPIC9K and newly built constitutive carrier pGAP9K, and electrotransformated the resultant expression plasmids into P… CONTINUE READING