[Fluorescent analysis of cryopreserved totipotent cells of amphibian embryos].

@article{Uteshev2002FluorescentAO,
  title={[Fluorescent analysis of cryopreserved totipotent cells of amphibian embryos].},
  author={Victor K Uteshev and E. V. Mel'nikova and Svetlana A Kaurova and Vladyslav Nikitin and Edith N Gakhova and Valery N. Karnaukhov},
  journal={Biofizika},
  year={2002},
  volume={47 3},
  pages={539-45}
}
The cryotolerance of totipotent cells from dissociated embryos of amphibian (grass frog Rana temporaria and grey toad Bufo bufo) was studied. Cell integrity and preservation of the cell barrier function were evaluated by fluorescent analysis. It was shown that the best cryopreservation of the cells was achieved by using the cryoprotective agent 10% dimethyl sulfoxide and 10% saccharose. These cells were successfully used for the homotransplantation of nuclei into enucleated eggs. The… CONTINUE READING