[Extraction and two-dimensional liquid chromatographic fractionation of plasma membrane proteome of mouse liver cells].

Abstract

OBJECTIVE To extract the plasma membrane proteins from mouse liver cells and investigate the approach for fractionating the protein mixtures by two-dimensional liquid chromatography. METHODS The plasma membrane of the liver cells from 10 mice was extracted by differential centrifugation and sucrose density-gradient centrifugation. The plasma membrane… (More)

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