[Expressions of ATF3 and CTGF and their regulation by estradiol in the prepuce of hypospadias patients].

@article{Zhou2009ExpressionsOA,
  title={[Expressions of ATF3 and CTGF and their regulation by estradiol in the prepuce of hypospadias patients].},
  author={Juan Zhou and Hao Wang and Yu-Bing Peng and Qi Chen and Jun Da and Zhong Wang and Li-Min Ma},
  journal={Zhonghua nan ke xue = National journal of andrology},
  year={2009},
  volume={15 12},
  pages={1075-80}
}
OBJECTIVE Estrogen is closely associated with hypospadias. The present study was to explore the molecular mechanism of hypospadias caused by estradiol. METHODS Fibroblasts obtained from the prepuce of hypospadiac and normal children were cultured in vitro and treated with 17-beta ethinyl estradiol (17-EE) at the concentrations of 1 micromol/L to 0.1 nmol/L for 2 hours, or at 0.1 micromol/L for 0.5, 1, 2, 4, 8, 16 and 24 hours. MTT assay was used to evaluate the effect of 17-EE on the… CONTINUE READING