[Expression of hemagglutinin of avian influenza virus (AIV) and its application in diagnosis of AIV H9 subtype].

@article{Zhang2005ExpressionOH,
  title={[Expression of hemagglutinin of avian influenza virus (AIV) and its application in diagnosis of AIV H9 subtype].},
  author={Rui-hua Zhang and Mei-lin Jin and Gui-hua Wang and Z Yu and Si-ting Zhao and Hong-Chao Li and Ya-Di Tan and Huan-chun Chen},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2005},
  volume={21 2},
  pages={315-9}
}
In order to differently diagnose avian influenza virus (AIV) subtypes, the HA gene of AIV H9 subtype was cloned, expressed and utilized in an enzyme-linked immunoad sorbent assay (ELISA). HA gene (1683bp) of H9N2 AIV was amplified by RT-PCR from a strain of field isolated H9N2 AIV, and its identity was confirmed by sequencing. The HA gene was subcloned into… CONTINUE READING