[Expression of full-length hepatitis E virus structural gene using baculovirus expression system].

OBJECTIVE To obtain recombinant baculovirus containing full-length structural gene of HEV and to express HEV structural protein. METHODS Full-length structural gene of HEV (5147-7126 nt) was inserted into baculovirus expression vector pAcUW51. The recombinant plasmid and Baculo Gold DNA were co-transfected into insect cell line Sf9. Single plaque was… CONTINUE READING