[Expression of a gene fragment from the alpha-subunit of the acetylcholine receptor from Torpedo californica in Saccharomyces cerevisiae].

@article{Teliakova1994ExpressionOA,
  title={[Expression of a gene fragment from the alpha-subunit of the acetylcholine receptor from Torpedo californica in Saccharomyces cerevisiae].},
  author={O V Teliakova and Iu N Utkin and V. I. Tsetlin and I V Severtsova and N M Zvonok},
  journal={Bioorganicheskaia khimiia},
  year={1994},
  volume={20 5},
  pages={546-50}
}
A fragment of the AChR alpha-subunit gene from Torpedo californica (about 800 bp) was joined in frame with a synthetic duplex, coding for a changed leader peptide of the Kluyveromyces lactis killer toxin under the control of the reconstructed glyceraldehyde-3-phosphate dehydrogenase gene promoter in the pUC8 vector. Translation termination codons in all… CONTINUE READING