[Expression, purification and antigenicity of P35 gene of Toxoplasma gondii].


OBJECTIVE P35 is an important surface protein for Toxoplasma gondii. To obtain the highly pure and specific antigenicity protein, the gene P35 was cloned and its product was expressed in E. coli Rosetta. The expressed protein was purified and its immunogenecity was studied. METHODS A pair of primers was designed according to cDNA sequence of P35, and then… (More)


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