[Expressing of porcine beta-defensin-2 mature peptide in the yeast].

  title={[Expressing of porcine beta-defensin-2 mature peptide in the yeast].},
  author={Han Hu and Binbin Yu and Quqiong He},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  volume={51 5},
OBJECTIVE To construct the recombinant Pichia pastoris KM71 in which Porcine beta-defensin-2(pBD-2) mature peptide could be stably expressed and the antimicrobial effect was evaluated. METHOD According to the requirement of yeast codon bias for protein expression, three pairs of primers were designed for development of SOE-PCR to amplify the genes coding pBD-2 mature peptide. The resultant genes were cloned into pPIC9k and pPIC9k-Gultathione S Transferase (GST) vectors to yield the… CONTINUE READING

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