[Experimental expression of HIF-1α in rabbit adipose-derived mesenchymal stem cells by dynamic compression plus IGF-1 gene transfection].

@article{Yang2010ExperimentalEO,
  title={[Experimental expression of HIF-1α in rabbit adipose-derived mesenchymal stem cells by dynamic compression plus IGF-1 gene transfection].},
  author={Jun Yang and Chuan-Hui Zhang and Jian-Jun Li and Zhen-dong Zhou and Qin-ming Zhang},
  journal={Zhonghua yi xue za zhi},
  year={2010},
  volume={90 45},
  pages={3220-4}
}
OBJECTIVE To investigate the effects on HIF-1α expression of rabbit adipose-derived mesenchymal stem cells (ADSCs) by dynamic compression plus IGF-1 gene transfection and explore the mechanism of promoting chondrogenesis by HIF-1α. METHODS The ADSCs were harvested after the digestion of typeIcollagenase and transfection with pcDNA3.1-IGF-1. And then the cells were seeded onto chitosan/gelatin scaffolds with a density of 5 × 10(7) cells/ml and divided into groups: Group A (control), non… CONTINUE READING