[Evaluation of the effect of the dilution of urine on the toxicity and isolation of cytomegalovirus using the rapid shell-vial centrifugation culture technique].


Working with highly passaged MRC-5 fibroblast monolayers in a shell-vial culture-centrifugation assay for rapid detection of cytomegalovirus (CMV), a direct toxic effect of the urine on the monolayer was observed in as many as 44% of cases when vials were inoculated with undiluted specimens. This toxicity was reduced to only 6% by inoculation of shell-vials… (More)


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