[Ethylene oxide sterilization. I. Influence of the sporulation medium on the resistance of spores of Bacillus subtilis var. niger].

Abstract

Some elements influencing the resistance of spores used in ethylene oxide sterilization process control are standardized. Spores of Bacillus subtilis var. niger were produced in chemically defined liquid and solid sporulation media to a total of 12 lots; after standardization of the number of spores, they were challenged by sub-lethal cycles, followed by a… (More)

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