[Establishment of eukaryotic cell line stably expressing soluble human leucocyte antigen G1 by nucleofection].

Abstract

OBJECTIVE To establish a eukaryotic cell line that can express soluble human leucocyte antigen G1 (sHLA-G1) stably. METHODS The recombinant plasmid pcDNA3-sHLA-G1 is transfected by a novel nonviral, electroporation-based gene transfer method termed nucleofection into the host cell lymphoblastoid cell line (LCL) 721.221 which does not express any HLA… (More)

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