[Establishment of BHK-21 cell lines stably expressing FMDV 3Dpol gene by retroviral-mediated gene transfer technique].

OBJECTIVE The aim of the study is to establish in vitro cell line with stable and effective 3Dpol gene expression, so as to study the biological function of foot-and-mouth disease virus (FMDV ) 3Dpol and foot-and-mouth disease (FMD) gene engineering vaccine. METHODS FMDV 3D gene was amplified from pMD18-T-3D and inserted into pGEM-Teasy vector. By NotI… CONTINUE READING