[Establishment and optimization of the regeneration system for common dandelion (Taraxacum officinale Weber)].

@article{Chen2005EstablishmentAO,
  title={[Establishment and optimization of the regeneration system for common dandelion (Taraxacum officinale Weber)].},
  author={Hua Chen and Ping Li and Jing Liu and Yin-Xin Li},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2005},
  volume={21 2},
  pages={244-9}
}
A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Multiple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For indirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerated plantlets were obtained when these calli were cultured on MS medium containing 1.0 mg/L 6-BA. Random amplified… CONTINUE READING

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