[Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells].

@article{Li2002EstablishmentAA,
  title={[Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells].},
  author={Hong-fan Li and Xing-rong Liu and Feng-yan Heng and Ning-hua Wu and Yu-fei Shen},
  journal={Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae},
  year={2002},
  volume={24 3},
  pages={321-4}
}
OBJECTIVE To establish a RT-PCR system for detecting mRNA expression level of 4 hsp genes in human cells. METHODS RT-PCR system was established with gene cloning, gene recombination, in vitro transcription and RT-PCR techniques; The detection of expression level of 4 hsp genes in SW13 cell was carried out with this system. RESULTS In SW13 cell, hsp70… CONTINUE READING