[Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody].

OBJECTIVE To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library. METHODS By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. RESULTS Twelve positive clones were chosen for DNA sequencing. From the… CONTINUE READING