[Enrichment and characterization of a bacteriolytic amoebic enzyme].

Abstract

Extracts of cultures of a small free-living amoeba, which feeds mainly on gram-negative bacteria, are capable of lysing heat-killed gram-negative bacteria. The lytic activity was enriched 36-fold by means of precipitation with (NH4)2S04 and of chromatography on Sephadex G-75, followed by ultrafiltration. The activity is less resistant to heat than is… (More)

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