[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells].

@article{He2003EffectsOT,
  title={[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells].},
  author={Jing Jing He and Genze Shao and Rouli Zhou},
  journal={Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences},
  year={2003},
  volume={35 4},
  pages={
          348-52
        }
}
OBJECTIVE To study the biological effects of the novel gene LAPTM4B high expressed in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells. METHODS Eukaryotic expression plasmid pcDNA3-TM4B was constructed and transfected into mouse NIH3T3 cell line using Lipofectamin-2000 mediating gene transfer technique. Monoclonal transfected cells with high expression of LAPTM4B gene were identified and selected by RT-PCR, Northern blot and Western blot method. Cell surface… CONTINUE READING

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