[Effects of methylation status of CpG islands on results of luciferase reporter assay].

@article{Zhang2009EffectsOM,
  title={[Effects of methylation status of CpG islands on results of luciferase reporter assay].},
  author={Bao-Zhen Zhang and Dajun Deng},
  journal={Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]},
  year={2009},
  volume={43 7},
  pages={601-6}
}
OBJECTIVE To investigate the effects of methylation status of CpG islands of endogenous E-cadherin (CDH1) gene on the promoter activity of corresponding genes in reporter assays. METHODS The methylation statuses of CpG island of CDH1 in 8 different cell lines were detected by methylation-specific PCR. CDH1 protein was analyzed by Western blotting. Two sets of pGL3 reporter vectors with different genotypes/haplotypes of the CDH1 promoter were constructed [ pGL3-A(-73)/-C(-73) pGL3-H1/-H4] and… CONTINUE READING